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Figure 2 | BMC Cell Biology

Figure 2

From: A novel fluorescence-based biosynthetic trafficking method provides pharmacologic evidence that PI4-kinase IIIα is important for protein trafficking from the endoplasmic reticulum to the plasma membrane

Figure 2

Treatment with brefeldin A inhibits the biosynthetic trafficking of EGFR to the PM. Live cells transiently expressing EGFR-GFP were processed as described for Figure 1, except that BFA (5 μg/ml) was added to half of the cells at hour 3. (A) Flow cytometry was used to calculate the ratio of PM localized EGFR fluorescence to total EGFR fluorescence, and this ratio for each time point ± BFA is plotted. Error bars indicate ± SE of four independent experiments in which approximately 8,000 EGFR-GFP expressing cells were analyzed at each time point. *** p < 0.001 (B) Confocal images of RBL cells expressing EGFR-GFP either untreated or treated with BFA, cells are from 8 hr time points quantified in A. Scale bars show 5 μm.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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