Protease 3C of hepatitis A virus induces vacuolization of lysosomal/endosomal organelles and caspase-independent cell death

Table 2 Primers used

Primer	Sequence*
Bi3Cf	GCAGAAGATATC GCCACCATGTCAACTCTAGAAATAGCAGGA
Bi3Cr	CACTTTGCTAGCTTACTGACTTTCAATTTTCTTATC
Bi3Cm-f	GGTCTTCCCGGGATGGCTGGTGGGGCCCTAGTG
Bi3Cm-r	CACTAGGGCCCCACCAGCCATCCCGGGAAGACC
rt-3Cf	GGTTCAGTTTGGAGTTGGTGA
rt-3Cr	TTCCTCTCCATGCCTGATCT
rt-GAPDHf	GGTCGTATTGGGCGCCTGGTCACC
rt-GAPDHr	CACACCCATGACGAACATGGGGGC

*Restriction sites are boldfaced, Kozak sequence is italic, sequence corresponding to Cys172 → Ala mutation is boldfaced and italized. All primers were from Evrogen (Russia).

ISSN: 2661-8850