Figure 2

PI3K/Akt inhibitor normalized MMT relevant proteins’ expression and peritoneal fibrosis in PD mice model. Panel A: Hematoxylin-Eosin (top panels) and Masson’s Trichrome staining (bottom panels) of the parietal peritoneum in control mice, PD mice with or without LY294002 treatment. Panel A1: Semi quantification of H & E staining for the thickness of the sub-mesothelial tissue of the parietal peritoneum. Panels B1 - B3: Bar graphs represent the mRNA expression of ZO-1, Vimentin and fibronectin (FN) in peritoneal tissues, as detected by real-time PCR. Panel C: Immunofluorescence microscopy of the peritoneal tissue sections following staining with ZO-1 (red) and Vimentin (green) antibodies. Treatment with LY294002 restored the expression of ZO-1 to a certain extent and inhibited the over-expression of Vimentin in the peritoneum compared to PD mice. Panel D: Western blot analysis showed the expression of ZO-1, Vimentin and FN. Panels D1-D3. Panels E1-E3: Bar graphs represent the densitometric measurements of the bands seen by Western blot analyses. Values are the mean ± SEM, n = 6, * p <0.01 vs. control group, # p <0.01 vs. PD group.