Figure 3
Expression of EMT/MMT relevant protein, fibronectin, smurf2, Smad7 and pAkt in HPMCs following TGF-β 1 treatment. Panel A: By Real-time PCR, a dose-dependent decrease in mRNA expression of ZO-1 was seen in HPMCs treated with TGF-β1, while an increased expression of Vimentin and Smurf2 miRNA was observed. Panel B: Western blot analyses showed that the protein expression of ZO-1 and Smad7 was decreased with a dose-dependent manner in HPMCs treated with TGF-β1. In contrast, the expression of Vimentin, pAkt and Smurf2 was significantly increased. Panel C: The bar graphs represent the densitometric measurements of the bands seen by Western blotting procedures. Panel D: The bar graph represents the fibronectin concentration in the supernatant of cultured HPMCs induced by TGF-β1 and detected By ELISA. Values are the mean ± SEM, n = 6, * p <0.01 vs. control.