Dexamethasone in osteogenic medium strongly induces adipocyte differentiation of mouse bone marrow stromal cells and increases osteoblast differentiation

Table 2 Primers sequences and conditions of PCR

Gene	Primer sequences	Annealing temperature	Product length	Genbank
Runx2	F: GCCGGGAATGATGAGAACTA	62°C	200 bp	NM_001146038.2
Runx2	R: GGACCGTCCACTGTCACTTT	62°C	200 bp	NM_001146038.2
OC	F: AAGCAGGAGGGCAATAAGGT	60°C	364 bp	NM_007541.3
OC	R: CGTTTGTAGGCGGTCTTCA	60°C	364 bp	NM_007541.3
OSX	F: CTAGTTCCTATGCTCCGACC	54°C	237 bp	NM_130458.1
OSX	R: TCATCACATCATCATCGTG	54°C	237 bp	NM_130458.1
ALP	F:CAAAGGCTTCTTCTTGCTGGT	60°C	257 bp	NM_007431.3
ALP	R: AAGGGCTTCTTGTCCGTGTC	60°C	257 bp	NM_007431.3
PPAR gamma −2	F: GGTGAAACTCTGGGAGATTCT	55°C	268 bp	NM_011146.3
PPAR gamma −2	R: CAACCATTGGGTCAGCTCTTG	55°C	268 bp	NM_011146.3
Leptin	F: CTCATGCCAGCACTCAAAAA	62°C	197 bp	NM_008493.3
Leptin	R: AGCACCACAAAACCTGATCC	62°C	197 bp	NM_008493.3
Glut4	F: ACTCTTGCCACACAGGCTCT	62°C	174 bp	NM_009204.2
Glut4	R: AATGGAGACTGATGCGCTCT	62°C	174 bp	NM_009204.2
Adiponectin	F: CCCAGTCATGCCGAAGA	62°C	354 bp	NM_009605.4
Adiponectin	R: TACATTGGGAACAGTGACGC	62°C	354 bp	NM_009605.4
GAPDH	F: GGCATTGCTCTCAATGACAA	62°C	200 bp	NM_008084.1
GAPDH	R: TGTGAGGGAGATGCTCAGTG	62°C	200 bp	NM_008084.1
18S	F: ATTCCGATAACGAACGAGAC	60°C	297 bp	NR_003278.3
18S	R: GCTTATGACCCGCACTTACT	60°C	297 bp	NR_003278.3
Cyclin D1	F: CGCACTTTCTTTCCAGAGTCA	55°C	74 bp	NM_007631.2
Cyclin D1	R: AAGGGCTTCAATCTGTTCCTG	55°C	74 bp	NM_007631.2
Cyclin E1	F: TCAACGACACGGGTGAG	55°C	196 bp	NM_007633.2
Cyclin E1	R: ATGTCGCACCACTGATAACCT	55°C	196 bp	NM_007633.2
Cyclin B1	F: CGCTCAGGGTCACTAGG	54°C	159 bp	NM_172301.3
Cyclin B1	R: CTTCGCTGACTTTATTACCAA	54°C	159 bp	NM_172301.3

ISSN: 2661-8850