Figure 1

Adipocyte- and osteoblast-specific mRNA and miRNA quantification in osteoblasts incubated with hMSC-0, 7, 14 and 21 days differentiated adipocyte medium. (A) RT-qPCR analysis of PPARγ, Leptin, CEBPα and CEBPδ in 14 days-differentiated osteoblasts incubated with conditioned medium from 0, 7, 14, and 21 days-differentiated adipocytes (hAdi-CM). Transcript levels of YWHAZ were used for sample normalization [29]. Data was obtained from 4 independent experiments (Bars: median ± interquartile space). Differences were considered significant at p < 0.05 (*p < 0.05). (B) RT-qPCR analysis of miR-138, miR-30c, miR-125a, miR-125b, miR-31 in 14 days-differentiated osteoblasts incubated with conditioned medium from 14 days-differentiated adipocytes (hAdi-CM). Transcript levels of RNU6P were used for sample normalization. Data was obtained from 3 independent experiments (Bars: median ± interquartile space). (C) RT-qPCR analysis of OC and OP in 14 days-differentiated osteoblasts incubated with conditioned medium from 0 (ctrl) or 14 days-differentiated adipocytes (hAdi D14-CM). Transcript levels of YWHAZ were used for sample normalization. Data was obtained from 3 independent experiments (Bars: median ± interquartile space). For (A), (B) and (C) control (ctrl) was performed by incubating hMSC-derived osteoblasts with serum- and inductors-free DMEM for the same time.