Figure 5

Specificity of proximal tubular cells enrichment. A) Density plot of prominin-1 expression showing an overlay of total kidney cell suspension in red (with a prominin-1 positive fraction estimated by flow cytometry at 37% of total cells in the suspension), prominin-1-depleted cells in orange (with a prominin-1 positive fraction estimated by flow cytometry at 15% of total cells in the suspension), and prominin-1 enriched cells in blue (with a prominin-1 positive fraction estimated by flow cytometry at 92% of total cells in the suspension). B) RT-PCR quantification of prominin-1, Cd31 and Aqp2 mRNAs in the eluted and the Prominin-1 enriched fractions. The results are shown as relative quantifications using the geometrical mean of gene Gusb and Rpl32 as the reference.