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Fig. 1 | BMC Cell Biology

Fig. 1

From: Effects of partial silencing of genes coding for enzymes involved in glycolysis and tricarboxylic acid cycle on the enterance of human fibroblasts to the S phase

Fig. 1

Levels of mRNAs of genes coding for glycolytic and tricarboxylic acid cycle enzymes in human dermal fibroblast cells treated with siRNAs. Cells were seeded in 6-well plates, and transfected with siRNAs. After 72 h incubation, total RNA was purified and the level of mRNA was estimated by qPCR analysis. Presented results are mean values from at least three independent experiments, with error bars indicating SD. In each experiment, mRNA level measured in untreated cells was used as a control value (100 %, dashed line). In all experiments, statistically significant differences (p < 0.05 in the t-test) were found relative to the control

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