Fig. 3
From: Distinct expression patterns of HCN channels in HL-1 cardiomyocytes
Expression of HCN channel isoforms in HL-1 cells. Immunostaining of HCN1 (a), HCN2 (b), and HCN4 (c) in confluent HL-1 cell cultures was performed with HCN isoform-specific primary antibodies (see Additional file 1: Table S1) followed by fluorescently labeled secondary antibodies. HL-1 cells were double-immunostained for HCN1 (d1) and HCN4 (d2) and for HCN2 (e1) and HCN4 (e2); merged images of double-immunostainings are shown in (d3) and (e3), respectively. Some cells specifically stained for either isoform are indicated by arrowheads; co-immunolabeled cells are marked by asterisks. Scale bars specify 20 μm