Fig. 5
From: Extracellular fluid flow and chloride content modulate H+ transport by osteoclasts
a. Emitted fluorescence of BCECF-loaded osteoclast-like cells (OCL-cells) individualized in the area defined by the colored circle line by using the MetaFluor 7.1 software (b). pHi of OCL-cells shown in (a). Each line represents one OCL-cell. At the time the flow is stopped (dashed line), pHi become progressively more alkaline, thus indicating H+ secretion since the standard HEPES-buffered solution does not contain HCO3 −. c. Transmitted light image of selected osteoclast. d. pHi of the osteoclast shown in c. At the time the flow is stopped (dashed line), pHi become progressively more alkaline, thus indicating H+ secretion since the standard HEPES-buffered solution does not contain HCO3 −