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Fig. 3 | BMC Cell Biology

Fig. 3

From: Dissecting seipin function: the localized accumulation of phosphatidic acid at ER/LD junctions in the absence of seipin is suppressed by Sei1pΔNterm only in combination with Ldb16p

Fig. 3

PA puncta appear with TAG and SE droplets in sei1Δ strains. Yeast strains chromosomally expressing Opi1-mCherry, with or without a SEI1 allele, and which only produce neutral lipid upon addition of galactose ((a) 3KO(GALDGA1) produces TAG, while (b) 3KO(GALARE1) produces steryl ester, SE [14]), were subjected to galactose at 0 time and monitored every three hours. PA puncta (marked by Opi1-mCherry) became apparent only in sei1Δ cells and only upon formation of lipid droplets, detected by BODIPY. PA puncta were present in both TAG and SE droplets

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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