Fig. 1
From: Adopting the rapamycin trapping assay to track the trafficking of murine MHC class I alleles, H-2Kb
Endogenous H-2Kb accumulate in a juxtanuclear region and can reach the cell surface. MEF cells were fixed, permeabilized, and double stained for H-2Kb with anti-P8 serum and for one of the early secretory organelles, either ER (anti-PDI), ERGIC (anti-p58), or cis-Golgi (GM130). Prior to fixation, cells were either kept at 37 °C or incubated for 2 h at 15 °C or 20 °C, panels b and c, respectively. Most of the cells showed, in addition to the ER pattern, a juxtanuclear accumulation (a, arrows) that partially overlapped with the ERGIC and cis-Golgi stains. Some of the cells also showed weak cell surface stain (asterisk). Nuclei were stained with Draq5 as depicted in blue. All samples are shown in a bright-field mode to delineate the overall structure of the stained cells. Region of interest (ROI) from selected cells is quantified and a scatter plot with pearson’s coefficient is given.Scale bars, 10 μm