Adopting the rapamycin trapping assay to track the trafficking of murine MHC class I alleles, H-2Kb

Table 1 DNA constructs

Name (restriction enzyme)	Sequence	Amplification purpose
EGh_FRB- H-2K^b_fwd (HindIII)	5’- cccAAGCTTcgggcccacactcgctg	H-2K^b in ss-EGFP- FRB
EGh_FRB- H-2K^b_rev (BamHI)	5’- gtGGATCCtcacgctagagaatgagg	H-2K^b in ss-EGFP- FRB
EGh_FRB-tpn_fwd (HindIII)	5’-cccAAGCTTcgggaccagaggcgatcg	Murine tapasin in ss-EGFP-FRB
EGh_FRB-tpn_rev (BamHI)	5’- gtGGATCCttactgtgacttctttgagt	Murine tapasin in ss-EGFP-FRB

EGFP-H-2K^b was generated as described in Garstka et al. 2007. The Cerulean-FKBP-Ii and Cerulean-FKBP-GPI constructs were obtained from Dr. Jennifer Lippincott-Schwartz (NIH, Bethesda, US). The other constructs, Cerulean-FKBP-p58, Venus-FRB-K^b, and Venus-FRB-Tapasin were generated using the following primers

ISSN: 2661-8850