Fig. 3
From: Direct Interaction between TalinB and Rap1 is necessary for adhesion of Dictyostelium cells
A TalinB mutant that does not interact with Rap1 cannot rescue development of talB-null cells. a Western blot with α-GFP antibody on Dictyostelium talA/B-null cell lysate expressing GFP-TalB or GFP-TalB-K16A. b Mutations in the RA domain of TalinB do not influence protein localization. Wild-type GFP-TalB and mutated GFP-TalB-K16A were overexpressed in talA/talB-null background and their localization was tested using confocal microscopy. Scale bar represents 10 μm. c Wild-type cells, talB-null cells and talB-null cells expressing wild-type GFP-TalB or mutated GFP-TalB-K16A were allowed to develop at non nutrient agar for 30 h; development of the cells was monitored by light microscopy. As previously shown talB-null cells are arrested in development at the mound phase. This defect cannot be rescued by the GFP-TalB-K16A mutant.