Fig. 1

Cold shock reduces longevity after initial recovery. a Characterization of the phenotypes of worms after 1 h of recovery at 20 °C following 2 °C cold shocks of different durations. Phenotype was determined by nose touch test. Those worms that only responded with small twitches of the nose or pharynx pumps without any motion of the body were classed as immobile. b Percent survival of worms during the 4 days following return to 20 °C after different durations of 2 °C cold shock. Sample sizes are as follows: 1-hour cold shock N = 158; 4-hour cold shock N = 166; 12-hour cold shock N = 483; 24-hour cold shock N = 150; 48-hour cold shock N = 91