Fig. 3

Comparison of TT formation capabilities of HeLa cells expressing different Cxs. A typical view of TT formation between HeLa Cx45 (a) and Cx36-EGFP cells (b). c Wt and each Cx-expressing HeLa cells were seeded in 24-well plates with glass coverslips on the bottom at equal densities (3 × 10⁴ in each well) and 36 h later were examined using differential interference contrast microscopy with × 20 lens. The experiments were repeated in 2 passages. The number of TTs was counted in 15 randomly selected regions per coverslip and presented as the average values per 1 mm² or per 100 cells (n = 2*15 = 30) (see Table 1). *p < 0.05