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Fig. 2 | BMC Cell Biology

Fig. 2

From: Altered cellular localization and hemichannel activities of KID syndrome associated connexin26 I30N and D50Y mutations

Fig. 2

Effect of Cx26I30N and D50Y mutations on protein trafficking. a Merged images of Cx26WT, I30N and D50Y transfected cells that were co-stained with Cx26 (red) and the Golgi apparatus marker, golgin-97 (green) antibodies. Blue is for DAPI staining of the nucleus. Arrow heads point the location of the Golgi apparatus. Yellow signals in Cx26I30N and D50Y images show the colocalization of Cx26 and golgin-97. Scale bar 10 μm. b Merged images of Cx26WT, I30N and D50Y transfected cells that were co-stained with Cx26 (green) antibody and Rhodamine labeled wheat germ agglutinin (WGA). Blue is for DAPI staining of the nucleus. Yellow signals shown by arrow heads point out the colocalization of Cx26 and WGA. Scale bar 10 μm

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