Fig. 2

GM6001 inhibits TGF-β1-induced EndoMT in HKGECs. a Morphological changes in HKGECs induced by TGF-β1 (10 ng/ml) in the presence or absence of GM6001 were examined using phase contrast microscopy. b Indirect immunofluorescence staining of VE-cadherin and α-SMA were performed in HKGECs cultured in Endothelial Cell Medium with TGF-β1 (10 ng/ml). c Respective western blot analysis and quantitation of CD31, VE-cadherin and α-SMA were performed in HKGECs treated with TGF-β1 (10 ng/ml) in the presence or absence of GM6001. β-actin was used as a loading control. Original magnification × 200. Image are representative and data are expressed as mean ± SEM with n ≥ 3 for each experimental group, *P < 0.05, **P < 0.01