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Fig. 4 | BMC Cell Biology

Fig. 4

From: Specific localization of nesprin-1-α2, the short isoform of nesprin-1 with a KASH domain, in developing, fetal and regenerating muscle, using a new monoclonal antibody

Fig. 4

Immunofluorescent staining for nesprin-1 in cultured myoblasts and myotubes. Individual microscopic fields are shown as paired images. The left image of each pair shows nesprin-1 antibody stain (green) and the right image of each pair shows the merged antibody (green) and DAPI (blue) nuclear stain. a mAb N1G-Ex130 stained the nuclei of control myoblasts and myotubes whereas mAb N1alpha2-1H2 stained the nuclear envelope of control myotubes but not myoblasts. b The mAbs did not stain nesprin-1 mutant myoblasts or myotubes. c Similar to the image seen with N1G-Ex130, mAb MANNES1A and also the mAbs targeted against exons 81-86 (N1G-7C8 shown), stained the nuclear rim of control myoblasts and myotubes

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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