Fig. 6

Immunofluorescent staining of fetal and mature human muscle for nesprin-1-α2 and nesprin-1-giant. a mAb N1alpha2-1H2 stained nuclei at the nuclear rim and gave less intense staining of cytoplasm in fetal muscle. b Nesprin-1-α2 was weakly stained in nuclei (white arrows) of mature muscle. Nesprin-1-α2 in mature muscle was much less obvious than that seen in fetal muscle. c Nesprin-1-giant (mAb N1G-Ex130) was found at the nuclear rim, with low level cytoplasmic stain, in fetal muscle. Nesprin-1-giant was also seen at the nuclear rim in adult skeletal muscle (d). Figure shows representative images of total of 3 fetal donors and 4 mature donors