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Table 1 Relative expression of mRNA of nesprin-1 isoforms and markers of differentiation in cultured human myoblasts and myotubes from control donors

From: Specific localization of nesprin-1-α2, the short isoform of nesprin-1 with a KASH domain, in developing, fetal and regenerating muscle, using a new monoclonal antibody

  Control 5 days Control 25 years Control 41 years Control 53 years Control 79 years
  Myoblast Myotube Myoblast Myotube Myoblast Myotube Myoblast Myotube Myoblast Myotube
N1-Giant 61.1 ± 11.8 (5) 130.0 ± 12.8 (6) 88.3 ± 12.3 (6) 206.3 ± 17.3 (5) 47.7 ± 4.6 (4) 113.7 ± 19.3 (4) 47.4 ± 6.7 (5) 172.4 ± 22.1 (4) 60.5 ± 10.1 (4) 212.4 ± 12.1 (5)
  P < 0.001 P < 0.001 P < 0.001 P < 0.001 P < 0.001
N1-β1 <1 (1) <1 (1) <1 (2) <1 (2) <1 (1) <1 (1) <1 (1) <1 (1) 1.1 (1) 1.2 (1)
N1-β2 <1 (1) 1.2 (1) <1 (3) 2.1 ± 0.8 (3) <1 (1) 2.7 (1) <1 (1) <1 (1) <1 (1) 1.2 (1)
N1-α1 nd (1) nd (1) nd (1) nd (1) nd (1) nd (1) nd (1) nd (1) nd (1) nd (1)
N1-α2 <1 (5) 71.0 ± 9.5 (4) <1 (3) 47.5 ± 9.3 (4) <1 (5) 22.0 ± 1.3 (5) <1 (5) 44.7 ± 9.1 (4) <1 (3) 47.4 ± 4.3 (5)
  P < 0.001 P < 0.001 P < 0.001 P < 0.001 P < 0.001
CKM <1 (3) 1158 ± 116 (3) <1 (3) 2847 ± 285 (3) <1 (3) 740 ± 186 (3) <1 (3) 1884 ± 104 (3) 2.1 ± 1.7 (3) 1362 ± 68 (3)
  P < 0.001 P < 0.001 P < 0.005 P < 0.001 P < 0.001
Desmin 2773 ± 174 (3) 16434 ± 157 (3) 6931 ± 471 (3) 16966 ± 710 (3) 3784 ± 459 (3) 7103 ± 692 (3) 6532 ± 605 (3) 14799 ± 798 (3) 2484 ± 478 (3) 21973 ± 1803 (3)
  P < 0.001 P < 0.001 P < 0.005 P < 0.001 P < 0.001
  1. Results expressed as: Mean ± SD (n), “n” = number of repeat measurements. nd = Not detected