Table 1 Relative expression of mRNA of nesprin-1 isoforms and markers of differentiation in cultured human myoblasts and myotubes from control donors
Control 5 days | Control 25 years | Control 41 years | Control 53 years | Control 79 years | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
Myoblast | Myotube | Myoblast | Myotube | Myoblast | Myotube | Myoblast | Myotube | Myoblast | Myotube | |
N1-Giant | 61.1 ± 11.8 (5) | 130.0 ± 12.8 (6) | 88.3 ± 12.3 (6) | 206.3 ± 17.3 (5) | 47.7 ± 4.6 (4) | 113.7 ± 19.3 (4) | 47.4 ± 6.7 (5) | 172.4 ± 22.1 (4) | 60.5 ± 10.1 (4) | 212.4 ± 12.1 (5) |
P < 0.001 | P < 0.001 | P < 0.001 | P < 0.001 | P < 0.001 | ||||||
N1-β1 | <1 (1) | <1 (1) | <1 (2) | <1 (2) | <1 (1) | <1 (1) | <1 (1) | <1 (1) | 1.1 (1) | 1.2 (1) |
N1-β2 | <1 (1) | 1.2 (1) | <1 (3) | 2.1 ± 0.8 (3) | <1 (1) | 2.7 (1) | <1 (1) | <1 (1) | <1 (1) | 1.2 (1) |
N1-α1 | nd (1) | nd (1) | nd (1) | nd (1) | nd (1) | nd (1) | nd (1) | nd (1) | nd (1) | nd (1) |
N1-α2 | <1 (5) | 71.0 ± 9.5 (4) | <1 (3) | 47.5 ± 9.3 (4) | <1 (5) | 22.0 ± 1.3 (5) | <1 (5) | 44.7 ± 9.1 (4) | <1 (3) | 47.4 ± 4.3 (5) |
P < 0.001 | P < 0.001 | P < 0.001 | P < 0.001 | P < 0.001 | ||||||
CKM | <1 (3) | 1158 ± 116 (3) | <1 (3) | 2847 ± 285 (3) | <1 (3) | 740 ± 186 (3) | <1 (3) | 1884 ± 104 (3) | 2.1 ± 1.7 (3) | 1362 ± 68 (3) |
P < 0.001 | P < 0.001 | P < 0.005 | P < 0.001 | P < 0.001 | ||||||
Desmin | 2773 ± 174 (3) | 16434 ± 157 (3) | 6931 ± 471 (3) | 16966 ± 710 (3) | 3784 ± 459 (3) | 7103 ± 692 (3) | 6532 ± 605 (3) | 14799 ± 798 (3) | 2484 ± 478 (3) | 21973 ± 1803 (3) |
P < 0.001 | P < 0.001 | P < 0.005 | P < 0.001 | P < 0.001 | ||||||