Fig. 4

Evaluation of copy number by multiplexed ddPCR. The figure describes the structure of a FACS-like plot obtained with multiplexed ddPCR analysed with the QuantaSoft software, as in version 1.2.10.0 (Bio-Rad, CA, USA). a The area in blue shows the droplets positive for either or both unstable chromosomes analysed. The area highlighted in yellow shows droplets positive for the assay of Chr 11, whilst the area shaded in pink shows the droplets positive for the other unstable chromosome analysed (8); (b) a similar plot where each droplet populations are annotated