Fig. 4

FMNL2 is required for 2-D migration in A375 melanoma cells. a A375 cells were transfected with either control siRNA duplex or duplexes targeting all FMNL2 isoforms. The transfected cells were grown to confluence in scratch wound assay chambers (see Experimental Procedures). Wound closure was followed at the indicated times by phase contrast live-cell microscopy. b Equivalent samples of cell lysates from (a) were subjected to SDS-PAGE and immunoblotted to detect FMNL2 expression with anti-FMNL2 antibody. The pan-FMNL2 blot was stripped and re-probed with anti α-tubulin as a loading control. c Quantification of the data shown in (a). The gap in the cellular monolayer is represented as % wound closure. N = 3, error bars = SEM. ** indicates P < 0.01 and *** indicates P < 0.001