Fig. 1

hif-1 and djr-1.1 mutants are highly resistant to EtBr. a hif-1 animals exposed to EtBr have no effect on fecundity over three generations, while wild type worms showed a significant decrease in brood size. Brood size of N2 (wild type), djr-1.1 and hif-1 animals in 25 μg/mL EtBr were counted for three generations: F1, F2 and F3. Relative fecundity is measured as the number of progeny grown to adulthood on EtBr divided by the number of progeny grown to adulthood in the absence of EtBr, and would include animals that arrest at the L3 stage. Animals with ruptured vulva were not counted. Asterisk marks denote significant difference of fecundity between P0 (normalized to 1.0) and the following generations for each strain (* denotes p < 0.05 and ** p < 0.01) using one-way Anova. b Concentration dependence of EtBr. N2, hif-1, vhl-1 and djr-1.1 mutant animals (1st generation) were grown on worm plates containing nematode growth media and various concentrations of EtBr (0, 25, 50 and 100 μg/mL). The number of worms that grew to adulthood was determined and plotted. To determine significant differences between strains, the data were subjected to two-way ANOVA comparing groups (different strains) over different concentrations of EtBr. All strains showed a significant difference compared to N2 (***p < 0.001). c-h Growth of strains on EtBr. N2, djr-1.1 and hif-1 worms (as labeled) four days after egg-laying in plates containing either no EtBr (c, e, g) or 50 μg/mL EtBr (d, f, h)