Fig. 3

p53 inactivation and lamin A/C suppression result in aneuploidy and complex karyotypes. Primary p53 knockout MOSE cells were transfected with control or siRNA (si-Lam A) to suppress lamin A/C expression. The cells were maintained and passaged for 2 months in culture, and then subjected to chromosome analysis. Chromosome number counting and cytogenetic analysis were performed in 50 metaphase spreads for each cell preparation. At least 10 chromosome spreads from each preparation were randomly selected and estimated for chromosome number, and 2 appropriate samples were used for karyotyping. a and b, 2 representative examples of chromosome spreads from p53 (-/-) and siRNA-lamin A/C-treated MOSE cells are shown. c and d, 2 examples of karyotyping from p53 (-/-) and siRNA-lamin A/C-treated cells are shown