Fig. 5

In NOX2-null mice, the abundance and sizes of Cx40- and Cx43-containing gap junctions are not altered in atria of mice exposed to intermittent hypoxia. Cx43 and Cx40 were localized by immunofluorescence in frozen sections of atria of NOX2-null mice treated with RA or IH. (A) Representative photomicrographs are shown for each of these tissues and conditions. Bar, 20 μm. (B) Graph depicts the quantitation of the abundance of Cx43- and Cx40-immunoreactivity in atrial samples. The abundances of Cx43 and Cx40 did not differ significantly between RA (black bars) and IH treated mice (grey bars; p >0.05, Student’s t test). (C) Graph depicts the quantitation of the sizes of atrial Cx43- and Cx40-containing immunoreactive objects in these samples. The sizes of Cx43 or Cx40 immunoreactive particles did not differ significantly between RA (black bars) and IH treated mice (grey bars p >0.05, Student’s t test). n = 14 for all treatments