Skip to main content

Table 2 Measurements of nuclear parameters in wild type and mutant ES cells. Embryonic stem cells were plated on gelatinized tissue culture dishes and treated with or without RA for 4 days

From: Nuclear envelope structural proteins facilitate nuclear shape changes accompanying embryonic differentiation and fidelity of gene expression

ES Nuclei Wild type lmna (−/−) emd (−/−) lmna (−/−);emd (−/−)
mean (μm ± s.d.) - RA (n = 57) + RA (n = 65) - RA (n = 50) + RA (n = 34) - RA (n = 49) + RA (n = 22) - RA (n = 48) + RA (n = 34)
x- dimension 9.33 ±2.34 7.73 ±1.43 9.67 ±1.9 8.01 ±2.28 8.75 ±1.3 7.38 ±2.62 8.48 ±2.11 6.60 ±1.72
y- dimension 12.94 ±1.90 8.97 ±1.53 14.27 ±2.32 12.36 ±3.37 12.80 ±1.83 12.60 ±3.03 14.87 ±4.04 11.27 ±3.22
z- dimension 4.97 ±0.95 7.50 ±1.11 4.95 ±0.88 6.88 ±1.62 4.99 ±0.94 5.42 ±1.09 5.59 ±0.90 5.753 ±1.2
Shape factor 0.34 0.62 0.42 0.40 0.40 0.43 0.42 0.44
Volume (μm3) 333 ± 109 271 ±83 368 ±89 313 ±128 297 ±101 289 ±178 370 ±132 223 ±102
P value *P < 0.0005 *P < 0.05 P = 0.810 *P < 0.0001
  1. Measurements were made as described in Methods for wild type, lmna (−/−), and/or emd (−/−) cells. For the undifferentiated stage (− RA), values were determined in Oct3/4-positive cells. In differentiated cells (+ RA), only Gata4-positive nuclei were measured. Since no Gata4-positive nuclei were detected in lmna (−/−);emd (−/−) cells following treatment with RA, Oct3/4-negative (differentiated) nuclei were measured. The values were mean of the indicated number of nuclei analyzed with standard derivation shown. Statistically significant differences in nuclear volumes are indicated by *P