Fig. 1

Intermittent PTH treatment and mechanical strain affected the protein level of PTHR1 in OCCM-30 cells. a PTH-cycle dependent changes in PTHR1 expression detected by western blot. In each cycle, for the first 6 h, the control group was treated with vehicle culture medium containing acetic acid, and the PTH group received PTH treatment. Then they were both cultured in fresh medium without acetic acid or PTH for 18 h. GAPDH was used as a loading control. b Densitometry of bands was performed and normalized to GAPDH. c Western blot analysis of PTHR1 and GAPDH levels in OCCM-30 cells in the presence of mechanical strain and intermittent PTH. The Strain + PTH group represented cells that accepted 18 h mechanical strain, followed by 3 cycles of intermittent PTH treatment. The strain group cells were treated with 18 h mechanical strain, followed by 3 cycles of vehicle culture medium. There was neither mechanical strain nor intermittent PTH treatment in the control group. GAPDH was used as an endogenous reference. The experiment was repeated 3 times and the representative result is shown. d The bands were quantified and normalized to GAPDH. The control group was set to 1. Bars represent means ± SD of triplicate measurements. * p < 0.05