Fig. 4

Intermittent PTH markedly increased expression of some cementogenesis-related biomarkers. a-d The relative mRNA levels of BSP, OCN, COL1 and Osx in vehicle or 0-3 cycles of intermittent PTH treatment groups were determined by qPCR. For the first 6 h in each 24 h cycle, OCCM-30 cells were treated with vehicle or PTH (50 ng/ml) medium, then cells were cultured in fresh medium for the last 18 h in each cycle. Total RNA was subjected to qPCR. Relative mRNA expression levels were normalized to GAPDH as an endogenous reference. e The protein levels of BSP, OCN, COL1 and Osx in vehicle or 3 cycles of intermittent PTH treatment groups were determined by western blot. f-i Densitometry of bands was performed and normalized to GAPDH and fold change was determined relative to the control group. The experiments were repeated 3 times. Bars represent mean ± SD. * p < 0.05