Fig. 4

Expression of PUMA upon overexpression of the MDM2 splice variants. Activation of PUMA after transfection with the splice variants by qPCR assay revealing relative mRNA levels of PUMA. MCF-7 cells transfected with pCMV-GFP (GFP-control), MDM2-A, -B and -C, sorted after 12 h based on GFP expression. Graphs show the untreated cells (purple bars) and the 24 h 1 μM doxorubicin (green bars). The experiment was repeated in triplicate, with three independent transfections. * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001