Fig. 3

Leptin Deficiency Alters HSCs That Are Purified by Retinoid-based FACS Isolation. a Ultrapure HSCs were obtained by retinoid-based FACS of Nycodenz-gradient purified HSCs from WT and ob/ob mice. P1 population contains ultrapure HSCs, P2 population contains high side scatter (i.e., large), retinoid-high cells and only exists in ob/ob HSC prep, and P3 population contains cell debris and dead cells (which are removed via a 2 h cell adherence step in our standard HSC preps) (b) RNA was isolated from both freshly FACS sorted P1 or P2 cells (d0) or 7 day cultured FACS-sorted cells (d7), and gene expression profiles were examined by qRT-PCR. Ɨ p < 0.05 vs WT, * p < 0.05 vs d0. # non-detectable