Fig. 5
From: ULK1 phosphorylates Sec23A and mediates autophagy-induced inhibition of ER-to-Golgi traffic
Identification of ULK1 phosphorylation sites on Sec23A. a Candidate ULK1 phosphorylation sites on Sec23A. b Various phospho-deficient mutants were tested for ULK1 phosphorylation. This experiment was similar to that in Fig. 3b with ULK1 activated, but in addition to wild type Sec23A, four alanine mutants of Sec23A were also used. c Phospho-deficient and phospho-mimicking mutants of Sec23A were analyzed for their morphology. Hela cells were transfected with wild type or indicated mutants of Myc-Sec23A and the Myc-Sec23A mutants were stained with anti-c-Myc antibody.Amino acid starvation was induced to cell for 2Â h before fixation and staining (lower rows). Cell in the non-starved condition was cultured in complete growth medium before fixation (upper rows)