Fig. 10

Effects of Cladosporol A and doxorubicin treatment on the expression of apoptosis related proteins. (a) Representative images of immunofluorescence analysis of effects of the expression of; antiapoptotic Bcl-2 and pro-apoptotic Bax by confocal microscopy (using 40× oil immersion lens) in human breast cancer (MCF-7) cells treated with Cladosporol A for 24 h (b). Statistical analysis of the expression of Bcl-2 and Bax. The relative fluorescence intensity was compared to the control group. Data are mean ± S.D. of three similar experiments; statistical analysis was done with **p < 0.01 and ***p < 0.001. c Western blot indicating the increase in bax and decrease bcl-2 protein expression after Cladosporol A treatment in concentration dependent manner