Fig. 6
a Nuclear morphology analysis of human breast cancer (MCF-7) cells (2 × 105/ml/well) using DAPI after treatment with different concentrations of Cladosporol A (5, 10, 20 μM) for 24 h and examined using fluorescence microscopy (40X). Paclitaxel (1 μM) was used as positive control. With increase in concentration of Cladosporol A there is significant increase in nuclear condensation and formation of apoptotic bodies (b). The effect of Cladosporol A on the exposure of phosphatidylserine (PS) in human breast cancer (MCF-7) cells after 24 h treatment was analysed. Phosphatidylserine exposure was assessed by the annexin-V/ propidium iodide assay, as described in methodology and analyzed by confocal microscopy (c). The percentage of cells in early and late stages of apoptosis obtained by analysis of the cell images (mean ± SD, 3 experiments), p*< 0.05 and **p < 0.01