Fig. 6

RT-PCR amplification of integrin subunits and Rap1A mRNAs in HL60 cells. RT-PCR amplification (a) and densitometric quantification (b) of α4, α5, α6, αL, αM, αX, β1, and β2 integrin subunits as well as Rap1A expression in HL60 cells treated with the vehicle (Con) and 50 nM VD plus 5 ng/mL TNFα (VD-TNF). Data from four independent experiments, normalized to GAPDH, are shown as means ± SD. HL60 cells in the control group likely express relatively large amounts of α4β1, α5β1, and α6β1, αLβ2 integrins and substantial amounts of αMβ2 and αXβ2 integrins, whereas HL60 cells in the VD-TNF group likely express relatively large amounts α4β1, α5β1, α6β1, αLβ2, αMβ2, and αXβ2 integrins. *p < 0.05, **p < 0.01