Fig. 9

Typical micrographs of HL60 cells from the VD-TNF group on EphA2-Fc or ephrin-A1-Fc-adsorbed surfaces. a HL60 cells from the VD-TNF group were cultured on a coverslip surface on which the regions adsorbed with Fc, EphA2-Fc or ephrin-A1-Fc and Fc plus Matrigel and those with Fc plus Matrigel appeared alternatively as stripes, and phase-contrast images were photographed. Bright/round (arrows) and dark/polymorphic cells (arrowheads) are present as the major and minor populations of cells, respectively, observed not only on the EphA2-Fc or ephrin-A1-Fc-adsorbed test surfaces but also on the control surface. b A series of time-lapse micrographs obtained at 2-min intervals in the ephrin-A1-Fc-adsorbed region showing the extension and retraction of a number of cytoplasmic protrusions from the cells (arrows and arrowheads)