Table 1 Primers and cycle numbers for RT-PCR amplification of mouse mRNAs
Mouse | Primer | Product size (bp) | Annealing temp. (°C) | Cycle number | |
|---|---|---|---|---|---|
CD115 | Forward | 5-GGGACAGCACGAGAATATAG-3’ | 590 | 53.0 | 26 |
Reverse | 5’-CACTCTGAACTGTGTAGACG-3’ | ||||
CD34 | Forward | 5’-ACAACCACAGACTTCCCCAA-3’ | 424 | 59.5 | 33 |
Reverse | 5’-ATTGGCCAAGACCATCAGCA-3’ | ||||
EphA1 | Forward | 5’-TCGAGCCTTACGCCAACTAC-3’ | 466 | 69 | 38 |
Reverse | 5’-CCGATCAGCAGAGCTATTCC-3’ | ||||
EphA2 | Forward | 5’-GAGTGTCCAGAGCATACCCT-3’ | 549 | 62.5 | 37 |
Reverse | 5’-GCGGTAGGTGACTTCGTACT-3’ | ||||
EpA3 | Forward | 5’-TGTTGGTGCTTGTGTTGCC-3’ | 440 | 57 | 38 |
Reverse | 5’-CATTTCTTGGTGCGGATGG-3’ | ||||
EphA4 | Forward | 5’-GGGCAGTGAATGGAGTGTCT-3’ | 448 | 61.4 | 38 |
Reverse | 5’-AGAATGACCACGAGGACCAC-3’ | ||||
EphA5 | Forward | 5’-CTCTGGACGTGCCTTCTC-3’ | 319 | 57 | 38 |
Reverse | 5’-TCCCCAGTCCTCCAGGAA-3’ | ||||
EphA6 | Forward | 5’-GTGCGGAGCTTGGCTATGTT-3’ | 373 | 61.4 | 38 |
Reverse | 5’-GAGCTGAAGGTGGTCTAGTG-3’ | ||||
EphA7 | Forward | 5’-ACGGGGGAAGAAACGATGTC-3’ | 544 | 61.4 | 38 |
Reverse | 5’-GCTTCCTCAAGTGTGGCAAC-3’ | ||||
EphA8 | Forward | 5’-TTGTGGGAGTGGAACTCGCT-3’ | 632 | 61.4 | 38 |
Reverse | 5’-CCTGTCCGTTCTGGTAATGC-3’ | ||||
EphA10 | Forward | 5’-ACTACCTAGAAACCGAGACC-3’ | 493 | 53.0 | 38 |
Reverse | 5’-GACACCTTGTAAAACCCTGG-3’ | ||||
ephrin-A1 | Forward | 5’-CATCATCTGCCCACATTACG-3’ | 463 | 60.0 | 37 |
Reverse | 5’-AGCAGTGGTAGGAGCAATAC-3’ | ||||
ephrin-A2 | Forward | 5’-GGTGAGCATCAACGACTACC-3’ | 464 | 61.4 | 38 |
Reverse | 5’-CTGACACTAGGAGCCCAGAA-3’ | ||||
ephrin-A3 | Forward | 5’-TCCGCACTACAACAGCTCAG-3’ | 504 | 62.5 | 38 |
Reverse | 5’-TAGGAGGCCAAGAGCGTCAT-3’ | ||||
ephrin-A4 | Forward | 5’-AAGATTCAGCGCTACACACC-3’ | 420 | 61.4 | 38 |
Reverse | 5’-GATCCTCCGACTTTGCACAT-3’ | ||||
ephrin-A5 | Forward | 5’-CGTGGAGATGTTGACGCTG-3’ | 585 | 57.0 | 38 |
Reverse | 5’-GGCTCGGCTGACTCATGTA-3’ | ||||
GAPDH | Forward | 5’-GACTCCACTCACGGCAAAT-3’ | 689 | 57.0 | 22 |
Reverse | 5’-TCCTCAGTGTAGCCCAAGAT-3’ | ||||