Fig. 1
From: Coral cell separation and isolation by fluorescence-activated cell sorting (FACS)
Coral cell gating strategy for population analysis by FACS. a) P. damicornis cell suspension analyzed using FACS ARIA II according to their intrinsic size (FSC) and granularity (SSC) properties on a log scale, exited by 488 nm laser. The gate separates cells from debris. Within the inserts are representative images of the sorted cells or debris populations. Blue scale bar = 20 μm. b) Gating on live cells out of the cell gate using 405 nm (450/50BP)- DAPI negative gate. The fluorescent gates are in two dimensions due to P. damicornis and Symbiodinium natural auto-fluorescence. c) After excluding debris and dead cells the 488 nm (755LP 780/60BP) channel was used to measure Symbiodinium auto-fluorescent in this Far-Red channel to differentiate cells with or without Symbiodinium