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Fig. 1 | BMC Cell Biology

Fig. 1

From: Reduction of pluripotent gene expression in murine embryonic stem cells exposed to mechanical loading or Cyclo RGD peptide

Fig. 1

The presence of cRGDfC peptide with and without compression down-regulated mESC pluripotent markers such as (a) Oct 4, (b) Sox 2 and (c) Nanog, while (d) Rex 1 gene expression was up-regulated. Application of compressive force on mESC-Col I constructs with free integrin receptor resulted in the down-regulation of all tested pluripotent markers. Mean ± SD, n = 3. (***;p < 0.0001; **;p < 0.001; *;p < 0.05). Z-stack images showing that cell viability in the presence and absence of cRGDfC was not significantly different (e) mESC-Col I, 83% ± 7 and (f) mESC-Col I + cRGDfC, 75.3% ± 2.5, Scale bar = 100 μm

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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