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Fig. 3 | BMC Cell Biology

Fig. 3

From: Reduction of pluripotent gene expression in murine embryonic stem cells exposed to mechanical loading or Cyclo RGD peptide

Fig. 3

Effect of cRGDfC peptide on mESC morphology and viability when cultured in a dish without the presence of collagen matrix. (a) mESC with LIF and (b) mESC without LIF remained attached and showed no visible differences in cell morphology; (c) mESC with LIF + cRGDfC and (d) mESC without LIF + cRGDfC formed round shaped aggregates and detached from the dish. Scale bar, 200 μm. The morphology of aggregates formed in the presence (e) or absence (f) of LIF was also examined using DAPI staining with 3D confocal imaging and reconstruction. Scale bar, 100 μm. (g) Cell viability significantly decreased in the presence of LIF and cRGDfC to 50% in comparison to mESC w/LIF

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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