Fig. 5

Inhibition of Nek2 significantly lengthens cilia when cilia are assembling and disassembling. 10 μM of the Nek2 inhibitor (rac-CCT250863) was added to the AurA transfections, cells were serum starved to stimulate cilia formation (assembling, left panels) followed by reintroduction of serum to induce cilia disassembly (disassembling, right panels). Cilia number (a) and length (b) were measured for a total of at least 300 cells. (c-e) show typical examples of long cilia in cells serum starved and exposed to the Nek2 inhibitor and stained for gamma tubulin (green), acetylated tubulin (red) and DNA (blue); (f) shows a control cell. Scale bar equals 10 μm. The means for cilia number and length ± SEM are shown in Table 1