Fig. 6

Following transfection of RPMI2650 cells with full length Smad7 sequence or short hairpin RNA directed against Smad7. a RT-PCR was done to estimate relative mRNA expression of Smad7 in different groups of cells; b Western blot analysis also supported the above findings; c Cell viability was significantly increased following suppression of miR-17-5p; however, it was significantly increased following suppression of both miR-17-5p and Smad 7 expressions despite treatment with LPS; d Apoptosis assay revealed that although suppression of miR-17-5p suppressed apoptosis, suppression of both miR-17-5p and Smad7 expressions led to promotion of apoptosis; e Western blot also supported the same. f Relative mRNA expressions of different inflammatory cytokines were decreased following suppression of miR-17-5p expression; however suppression of both miR-17-5p and Smad7, led to increased mRNA expression of different cytokines; g-j Elisa estimated exact amount of the said inflammatory markers. Smad7: Mothers against decapentaplegic homolog 7; LPS: Lipopolysaccharides. * P < 0.05; **P < 0.01