Fig. 3

Glycine residues in the HIV-1 Env TMD do not affect its intracellular targeting. a Fusion proteins composed of the extracellular domain of the Tac protein, the transmembrane domain of Env protein (TMD) and a short cytosolic tail (CYT) are derived from the T-E26 chimera. In T-E39 the arginine present in the Env TMD is mutated to leucine. In T-E59 glycine residues in the Env TMD are mutated to leucines. Both mutations are combined in T-E60 (see also Table 1). b The fraction of each chimeric protein present at the cell surface was determined as described in the legend to Fig. 2. Statistical analysis revealed no significant differences from T-H0 (n = 3; one-way analysis of variance: p = 0.246)