Fig. 1

Subcellular localization of TGAT variants and quantification of their plasma membrane localization. a Schematic overview of TGAT fused to a fluorescent protein at its N-terminus. The blowout shows the last 20 amino acids of the C-tail of TGAT, with the last 15 amino acids indicated by the rectangle. Positively charged amino acids are shown in bold, amino acids with hydrophobic side-chains are underlined and the two cysteines at position 242 and 253 marked with an asterisk. b Confocal microscopy images of HeLa cells co-transfected with YFP-TGAT and the Golgi marker CFP-Giantin. The color overlay was generated by using the TGAT image for the green channel and the Golgi image for the blue and red channel (=magenta). Consequently, colocalized objects appear as white (right). c Confocal microscopy images of HeLa cells transfected with YFP, YFP-TGAT, YFP-TGAT^Δ15, YFP-TGAT^C242S, YFP-TGAT^C253S, YFP-TGAT^{C242S, C253S}. Width of the individual images is 60 μm in (b) and 110 μm in (c)