Fig. 7
Recruitment of the TGATΔ15 to several subcellular locations results in RhoA activation. a Hela cells transfected with the DORA-RhoA biosensor, Lck-FRB-ECFP(W66A) (plasma membrane) and RFP-FKBP12-TGATΔ15 (n = 23) or RFP-FKBP12 (control, n = 18) were stimulated with Rapamycin (100 nM) at t = 32 s. b Hela cells transfected with the DORA-RhoA biosensor, FRB-ECFP(W66A)-CAAX(RhoA) and FKBP12-RFP-TGATΔ15 (n = 15) or RFP-FKBP12 (control, n = 16) were stimulated with Rapamycin (100 nM) at t = 32 s. c Hela cells transfected with the DORA-RhoA biosensor, MoA-FRB-CFP(W66A) (mitochondria) and RFP- FKBP12-TGATΔ15 (n = 22) or RFP-FKBP12 (control, n = 21) were stimulated with Rapamycin (100 nM) at t = 32 s. d Hela cells transfected with the DORA-RhoA biosensor, Giantin-FRB-CFP(W66A) (Golgi apparatus) and RFP- FKBP12-TGATΔ15 (n = 21) or RFP-FKBP12 (control, n = 15) were stimulated with Rapamycin (100 nM) at t = 32 s. Time traces show the average ratio change of YFP/CFP fluorescence (±s.e.m)