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Fig. 6 | BMC Cell Biology

Fig. 6

From: Cyclic AMP signaling in Dictyostelium promotes the translocation of the copine family of calcium-binding proteins to the plasma membrane

Fig. 6

Pre-treatment, higher concentration of EGTA, and actin depolymerization does not change CpnF binding to membranes. a Cells expressing GFP-CpnF and CpnF-GFP were either disrupted in the presence of EGTA and calcium (pre-treatment) or disrupted and then treated with EGTA (10 mM) and calcium (2 mM). All membranes were pelleted by ultracentrifugation. The supernatant samples (S) and pelleted membranes samples (P) were analyzed by Western blot with an antibody to GFP. b Cells expressing GFP-CpnF were treated with Latrunculin A (LatA) or DMSO (control) for 30 min prior to disruption. All membranes were pelleted by ultracentrifugation. The supernatant samples (S) and pelleted membranes samples (P) were analyzed by Western blot with an antibody to GFP (α-GFP) and an antibody to actin (α-actin)

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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