Fig. 2
From: Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus
Intracellular detection of bacterial pathogens in exfoliative sulcular keratinocytes. a and b Representative images of exfoliative epithelial keratinocytes stained with Pap (a) and May-Giemsa (b) staining. Arrows, intracytoplasmic bacterial particles. Bar = 50 μm. c Immunocytochemical evaluation of LBP expression in the exfoliative sulcular keratinocytes. LBP antibody staining showed red-colored positive particles in the cytoplasm of sulcular cells. The nucleus was stained with Hoechst 33342 (blue). Arrows, intracytoplasmic bacterial particles. Bar = 50 μm. d Quantitative analysis of percentage of LBP-positive cells in exfoliative sulcular keratinocytes. The mean number of percentage of LBP-positive cells ± SD from five independent studies. *Significantly different at P < 0.05 compared with exfoliative cells from the free gingiva. e Western blotting analysis of LBP expression in exfoliative keratinocytes from gingival sulcus and free gingiva. β-actin (ACTB) was used as a loading control. Band densities were presented as LBP expression fold-increases (normalized to ACTB) and compared with the results for exfoliative cells from free gingiva. Quantification results are shown below the corresponding blots. All experiments were performed in quadruplicate