Fig. 4

Effect of incubation with IC50/48 h 2-BP on Trypanosoma cruzi epimastigotes as assessed by flow cytometry. a Forward scatter (FSC) analysis of control (CTL) and treated parasites, showing the larger size of the treated epimastigotes. b Side scatter (SSC) analysis of CTL and 2-BP parasites, showing an increase in granularity. c Cell cycle analysis, showing no difference between CTL and 2-BP treated parasites in the different cell cycle stages. d Mitochondrial potential analysis by rhodamine-123, showing a decrease in the membrane potential for 2-BP-treated epimastigotes; 100 μM CCCP: positive control. e Cell viability analysis of CTL and 2-BP parasites by propidium iodide, showing a few dead cells in 2-BP-treated cultures