Fig. 4
From: Acetylation of C-terminal lysines modulates protein turnover and stability of Connexin-32
C-terminal lysines modulate Cx32 ubiquitination. a Ubiquitination levels of 5R, and 5Q Cx32, relative to WT, was assessed by probing Western blots of Co2+ affinity-purified Cx32-Myc/6xHis with antibodies against pan-ubiquitin and Myc-tag. Note that monoubiquitin has a molecular mass of 8.5 kDa and thus the bands shown here could represent polyubiquitinated and multi-mono-ubiquitinated Cx32. b Densitometric quantification of Ub-Cx32 as a percentage of WT Ub-Cx32 is shown here. Relative levels of Ub-Cx32 were 47% and 15% for 5R and 5Q, respectively, versus WT (N = 3 experiments, Student’s T-test: * p < 0.05)