Fig. 3

Tan IIA promotes mitochondrial injury. a-b Mitochondrial ROS production was measured via flow cytometry using MitoSOX™ Red Mitochondrial Superoxide Indicator. Tan IIA at 1 μ M and 5 μ M were incubated with SW837 cells for 12 h. c-e The antioxidant factors, including GSH, SOD and GPX were quantified via ELISA in SW837 cells treated with Tan IIA. f-g Mitochondrial potential was observed using JC-1 staining and quantified by evaluating the ratio of red-to-green fluorescence intensity. h-m The alterations of mitochondrial proapoptotic and antiapoptotic proteins were detected using Western blotting. n-o Immunofluorescence assay for Smac. Blue fluorescence is the DAPI probe, which stained the nucleus. *p < 0.05